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gene screen nylon membranes  (Bio-Rad)


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    Bio-Rad gene screen nylon membranes
    Gene Screen Nylon Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+screen+nylon+membranes/gene+screen+nylon+membranes/pmc04591967-87-10-17
    Average 90 stars, based on 1 article reviews
    gene screen nylon membranes - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Human Papillomaviruses Modulate Expression of MicroRNA 203 upon Epithelial Differentiation to Control Levels of p63 Proteins
    Article Snippet: DNA was transferred to Gene Screen nylon membranes (Bio-Rad) using vacuum transfer according to the manufacturer’s protocols.

    Polymerase Chain Reaction:

    Article Title: Declining trend of Plasmodium falciparum dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) mutant alleles after the withdrawal of Sulfadoxine-Pyrimethamine in North Western Ethiopia
    Article Snippet: .. The denatured PCR product was blotted by direct application onto Gene screen nylon membranes using dot blotter (Bio-Rad, UK). ..

    Agarose Gel Electrophoresis:

    Article Title: The JAK-STAT Transcriptional Regulator, STAT-5, Activates the ATM DNA Damage Pathway to Induce HPV 31 Genome Amplification upon Epithelial Differentiation
    Article Snippet: .. The DNA samples were electrophoresed in a 0.8% agarose gel at 40 V overnight, and then transferred to Gene Screen nylon membranes (Bio-Rad, Hercules, CA) using vacuum transfer according to the manufacturer's protocols. ..



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    IRF-1 transcript level in uninduced and galactose-induced diploid yeast cells containing an IRF-1 expression plasmid. A–C: Diploid cells contained the IRF-1 expression plasmid YEpGIR and the YEpUAS reporter (Fig. 1B) with (A) VREαl, (B) VREβ”, (C) (GAAAGT)4. In the control sample (D), the diploid cells contained Z669 and YGRA2. Cells were grown for 24–38 hours in galactose (GAL) or glucose (GLU) medium, and the RNA extracted according to Vijayraghavan et al. (1989). Samples (5 μg) were run through a 1 % agarose gel. Endogenous ribosomal RNA was used as molecular weight marker. The samples were transferred to <t>Gene</t> <t>Screen</t> <t>nylon</t> <t>membranes</t> (DuPont) and hybridized as described (Büeler et al., 1992). The probe was the 1141 bp Nco I fragment of IRF-1 cDNA (Miyamoto et al., 1988) labeled by random priming (Feinberg and Vogelstein, 1983) and used at 3.5 × 105 cpm/ml. Autoradiography was for 1 hour.
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    Image Search Results


    IRF-1 transcript level in uninduced and galactose-induced diploid yeast cells containing an IRF-1 expression plasmid. A–C: Diploid cells contained the IRF-1 expression plasmid YEpGIR and the YEpUAS reporter (Fig. 1B) with (A) VREαl, (B) VREβ”, (C) (GAAAGT)4. In the control sample (D), the diploid cells contained Z669 and YGRA2. Cells were grown for 24–38 hours in galactose (GAL) or glucose (GLU) medium, and the RNA extracted according to Vijayraghavan et al. (1989). Samples (5 μg) were run through a 1 % agarose gel. Endogenous ribosomal RNA was used as molecular weight marker. The samples were transferred to Gene Screen nylon membranes (DuPont) and hybridized as described (Büeler et al., 1992). The probe was the 1141 bp Nco I fragment of IRF-1 cDNA (Miyamoto et al., 1988) labeled by random priming (Feinberg and Vogelstein, 1983) and used at 3.5 × 105 cpm/ml. Autoradiography was for 1 hour.

    Journal: Gene Expression

    Article Title: Interferon regulatory factor-1 (IRF-1) activates the synthetic IRF-1-responsive sequence (GAAAGT) 4 in Saccharomyces cerevisiae

    doi:

    Figure Lengend Snippet: IRF-1 transcript level in uninduced and galactose-induced diploid yeast cells containing an IRF-1 expression plasmid. A–C: Diploid cells contained the IRF-1 expression plasmid YEpGIR and the YEpUAS reporter (Fig. 1B) with (A) VREαl, (B) VREβ”, (C) (GAAAGT)4. In the control sample (D), the diploid cells contained Z669 and YGRA2. Cells were grown for 24–38 hours in galactose (GAL) or glucose (GLU) medium, and the RNA extracted according to Vijayraghavan et al. (1989). Samples (5 μg) were run through a 1 % agarose gel. Endogenous ribosomal RNA was used as molecular weight marker. The samples were transferred to Gene Screen nylon membranes (DuPont) and hybridized as described (Büeler et al., 1992). The probe was the 1141 bp Nco I fragment of IRF-1 cDNA (Miyamoto et al., 1988) labeled by random priming (Feinberg and Vogelstein, 1983) and used at 3.5 × 105 cpm/ml. Autoradiography was for 1 hour.

    Article Snippet: The samples were transferred to Gene Screen nylon membranes (DuPont) and hybridized as described ( Büeler et al., 1992 ).

    Techniques: Expressing, Plasmid Preparation, Control, Agarose Gel Electrophoresis, Molecular Weight, Marker, Labeling, Autoradiography